The Network of Antigen-Antibody Reactions in Adult Women with Breast Cancer or Benign Breast Pathology or without Breast Pathology

The Immunoglobulin G (IgG) antibody response to different protein antigens of the mammary ductal carcinoma by adult women affected by Breast Cancer (BC) distinguishes at least 103 proteins that differ in their molecular weights (MW). The IgG producing cell clones (nodes) coexist with each other in each individual organism and share energy resources among themselves, as well as factors that control the level of expression and Specificity of their IgG antibodies. So, it can be proposed that among them there is a Network of interconnections (links) unveiled by the antigens, which specifically react with the IgG antibodies produced by the clones. This Network possibly regulates IgG antibodies' activity and effectiveness. We describe the Network of nodes and links that exists between the different antigens and their respective IgG producing cell clones against the extracted protein antigens from the cells of the T47D Cell-Line, in 50 women with BC, 50 women with Benign Breast Pathology (BBP) and 50 women without breast pathology (H). We have found that women with BBP have the highest number of Links, followed by the H group and, lastly, the women with BC, a finding which suggests that cancer interferes with the Connectivity between the IgG producing cell clones and blocks the expression of 322 links in women with BBP and 32 links in women with H. It is also plausible that the largest number of links in the women with BBP indicates the Network’s state of arousal that provides protection against BC. On the other hand, there were many missing links in the BC group of women; the clone which lost more links in the BC group was the hub 24, which point to some of the antigens of T47D as potentially useful as vaccines, as the immune system of women with BBP is well aware of them.

针对罹患乳腺癌（Breast Cancer, BC）的成年女性，其针对乳腺导管癌不同蛋白抗原的免疫球蛋白G（Immunoglobulin G, IgG）抗体应答，可区分出至少103种分子量（Molecular Weight, MW）各异的蛋白质。产IgG的细胞克隆（nodes，节点）在个体体内彼此共存，不仅共享能量资源，还共享调控其IgG抗体表达水平与特异性的相关因子。据此可推测，这些克隆之间存在由抗原所揭示的相互连接网络（links），该网络可与克隆产生的IgG抗体发生特异性结合。此类网络或可调控IgG抗体的活性与效能。本研究描述了50名乳腺癌患者、50名乳腺良性病变（Benign Breast Pathology, BBP）患者以及50名无乳腺病变的健康女性（H）体内，针对从T47D细胞系（T47D Cell-Line）细胞中提取的蛋白抗原，不同抗原与其对应产IgG细胞克隆之间所存在的节点与连接网络。研究结果显示，乳腺良性病变组的连接数量最多，其次为健康对照组，乳腺癌组最少。该发现提示癌症会干扰产IgG细胞克隆间的连接连通性，并使得乳腺良性病变组的322个连接、健康对照组的32个连接无法正常表达。此外，乳腺良性病变组拥有最多的连接数，这或许意味着该免疫网络处于激活状态，可提供针对乳腺癌的保护作用。另一方面，乳腺癌组存在大量缺失的连接；乳腺癌组中丢失连接最多的克隆为枢纽（hub）24，这表明T47D的部分抗原可作为潜在疫苗候选，因为乳腺良性病变患者的免疫系统已对这些抗原充分识别。