Data_Sheet_1_Quick and wide-range taxonomical repertoire establishment of the cystic fibrosis lung microbiota by tandem mass spectrometry on sputum samples.xlsx

Microorganisms proteotyping by tandem mass spectrometry has been recently shown as a powerful methodology to identify the wide-range taxonomy and biomass of microbiota. Sputum is the recommended specimen for routine microbiological monitoring of Cystic Fibrosis (CF) patients but has been rarely submitted to tandem mass spectrometry-based proteotyping. In this study, we compared the microbial components of spontaneous and induced sputum samples from three cystic fibrosis patients. Although the presence of microbial proteins is much lower than host proteins, we report that the microbiota’s components present in the samples can be identified, as well as host biomarkers and functional insights into the microbiota. No significant difference was found in microorganism abundance between paired spontaneous and induced sputum samples. Microbial proteins linked to resistance, iron uptake, and biofilm-forming ability were observed in sputa independently of the sampling method. This unbiased and enlarged view of the CF microbiome could be highly complementary to culture and relevant for the clinical management of CF patients by improving knowledge about the host-pathogen dynamics and CF pathophysiology.

近年来，基于串联质谱（tandem mass spectrometry）的微生物蛋白质分型技术已被证实为一种可精准鉴定微生物群广泛分类学特征与生物量的高效方法。痰液是囊性纤维化（Cystic Fibrosis, CF）患者常规微生物监测的推荐样本，但基于串联质谱的蛋白质分型技术极少应用于痰液样本的相关分析。本研究针对3名囊性纤维化患者的自然咳痰与诱导咳痰样本的微生物组成展开对比分析。尽管样本中微生物蛋白的丰度远低于宿主蛋白，但本研究证实，可同时鉴定样本中的微生物群组分、宿主生物标志物，并实现对微生物群的功能解析。配对的自然咳痰与诱导咳痰样本之间，微生物丰度无显著差异。无论采用何种采样方式，痰液样本中均可检测到与耐药性、铁摄取及生物膜形成能力相关的微生物蛋白。这种对囊性纤维化微生物组的无偏且全面的解析视角，可与传统培养法形成极强的互补性；通过深化对宿主-病原体动态互作及囊性纤维化病理生理学的认知，该方法对囊性纤维化患者的临床管理具有重要参考价值。