Aluminum stress study in wheat and wheat-rye addition lines 3R and 6R.

Transcriptional profiling of three different genotypes (wheat cv Chinese Spring, and the wheat-rye addition lines 3R and 6R) comparing control and 24h exposure to 200 μM de AlCl3. The goal was to determine the effects of AlCl3 on global gene expression in each genotype and study the differences between them. Two-condition experiment (control vs aluminum exposure), for three genotypes (wheat cv Chinese Spring, wheat-rye addition line 3R, wheat-rye addition line 6R). Biological replicates: 3 control replicates, 3 aluminum exposed replicates. Please note that the experiments have been performed technically as dual channel (eg, Cy3 and Hy5-labeled samples hybridized to the same array) but processed as though they are single channel. Therefore there are total 9 raw data files for 18 samples and the corresponding raw data file (for each samples) is indicated in the sample description field. ***Please note that the 3R and 6R lines are called 'addition lines'. They are composed of the complete genome of Triticum aestivum + a pair of chromosomes from secale cereale (3R or 6R).****

本数据集针对3种不同基因型材料开展转录谱分析：小麦品种中国春，以及小麦-黑麦附加系3R和6R，比较了对照组与200μM氯化铝（AlCl3）暴露24小时的样品差异。本研究旨在明确氯化铝对各基因型全局基因表达的影响，并解析不同基因型间的表达差异。

本实验为双处理条件对照实验（对照组 vs 氯化铝暴露组），涵盖3种基因型：小麦品种中国春、小麦-黑麦附加系3R、小麦-黑麦附加系6R。生物学重复设置为：对照组3次生物学重复，氯化铝暴露组3次生物学重复。

请注意，本实验在技术层面采用双通道标记策略（例如，将Cy3与Hy5标记的样品杂交至同一基因芯片），但后续数据分析按照单通道模式进行。因此18个样品对应9个原始数据文件，每个样品对应的原始数据文件信息已在样品描述字段中注明。

***请注意，3R和6R品系被称为“附加系”，其基因组由普通小麦（Triticum aestivum）的完整基因组加上一对黑麦（Secale cereale）的3R或6R染色体组成。***