EFFECT OF PROBIOTIC ORAL ADMINISTRATION ON SKIN WOUND HEALING IN RATS

ABSTRACT Background: Manipulating intestinal microbiota with probiotics might stimulate skin response. Understanding all stages of the healing process, as well as the gut-skin-healing response can improve the skin healing process. Aim: To evaluate the effect of perioperative oral administration of probiotics on the healing of skin wounds in rats. Methods: Seventy-two Wistar male adult rats were weighed and divided into two groups with 36 each, one control group (supplemented with oral maltodextrin 250 mg/day) and one probiotic group (supplemented with Lactobacillus paracasei LPC-37, Bifidobacterium lactis HN0019, Lactobacillus rhamnosus HN001, Lactobacillus acidophilus NCFM® at a dose of 250 mg/day), both given orally daily for 15 days. The two groups were subsequently divided into three subgroups according to the moment of euthanasia: in the 3rd, 7th and 10th postoperative days. Results: There were no significant changes in weight in both groups. Wound contraction was faster in probiotic group when compared to the controls, resulting in smaller wound area in the 7th postoperative day. As for histological aspects, the overall H&E score was lower in the probiotic group. The probiotic group showed increased fibrosis from 3rd to the 7th postoperative day. The type I collagen production was higher in the probiotic group at the 10th postoperative day, and the type III collagen increased in the 7th. Conclusion: The perioperative use of orally administrated probiotic was associated with a faster reduction of the wound area in rats probably by reducing the inflammatory phase, accelerating the fibrosis process and the deposition of collagen.

摘要
背景：通过益生菌调控肠道菌群，或可激发皮肤应答反应。明晰愈合进程的全阶段以及肠-皮肤-愈合应答通路，有助于优化皮肤愈合效果。
目的：评估围手术期口服益生菌对大鼠皮肤伤口愈合的影响。
方法：选取72只成年雄性Wistar大鼠，称重后随机分为两组，每组各36只。对照组每日经口给予250mg麦芽糊精，益生菌组每日经口给予由副干酪乳杆菌（Lactobacillus paracasei）LPC-37、乳双歧杆菌（Bifidobacterium lactis）HN0019、鼠李糖乳杆菌（Lactobacillus rhamnosus）HN001、嗜酸乳杆菌（Lactobacillus acidophilus）NCFM®组成的复合制剂，给药剂量为每日250mg，两组均连续给药15天。随后根据安乐死时机将两组各划分为3个亚组，分别于术后第3、7、10天实施安乐死。
结果：两组大鼠的体重均未出现显著变化。与对照组相比，益生菌组的伤口收缩速率更快，术后第7天时伤口面积更小。组织学层面，益生菌组的整体苏木精-伊红（Hematoxylin-Eosin, H&E）评分更低。术后第3天至第7天，益生菌组的纤维化程度显著升高。术后第10天时，益生菌组的I型胶原表达水平更高，而术后第7天时其III型胶原表达量有所提升。
结论：围手术期口服益生菌可加快大鼠伤口面积的缩减速度，其潜在作用机制可能为缩短炎症阶段、加速纤维化进程并促进胶原沉积。