Human Blood Brain Barrier treated by Insulin

This study aims to map the dynamics of insulin-responsive pathways in polarized human cerebral microvascular endothelial cell (hCMEC/D3) monolayers, a widely used BBB cell culture model, to elucidate molecular mechanisms underlying BBB dysfunction in AD. RNA-Sequencing (RNA-Seq) was performed on hCMEC/D3 monolayers with and without insulin treatment at various time points.

本研究旨在绘制极化人脑微血管内皮细胞（hCMEC/D3）单层培养物中胰岛素响应通路的动态变化图谱，该模型为目前广泛应用的血脑屏障（Blood-Brain Barrier）细胞培养模型，以阐明阿尔茨海默病（Alzheimer's Disease, AD）血脑屏障功能障碍的潜在分子机制。本研究对经胰岛素处理与未处理的hCMEC/D3单层细胞在多个时间节点下开展了RNA测序（RNA-Seq）实验。