Concentrations of CPAs in ES1, ES2, and VS.

Coral reefs, which are crucial to the health and diversity of the global marine ecosystem, have experienced substantial declines due to climate change and human activities. Cryopreservation, the long-term storage of biological material at ultra-low temperatures, represents a novel strategy for coral conservation. The current study investigated the effects of integrating specific liposomes into vitrification solutions (VS) on the viability of oocytes from two coral species, oocytes were vitrified using a VS containing one of eight lipids. The study results indicate that incorporating PE and erucic acid considerably enhances the viability of J. juncea oocytes, whereas incorporating PC reduces their viability. However, incorporating palmitoleic acid, docosahexaenoic acid, linoleic acid, and eicosatrienoic acid did not substantially affect the viability of J. juncea oocytes. In J. fragilis, erucic acid yielded the highest viability (54.12% ± 2.99%), with oleic acid and docosahexaenoic acid also showing positive effects. Among the tested liposomes, erucic acid led to the highest overall viability in both species. Lipid-enhanced vitrification can assist coral conservation by facilitating cryobanking of coral gametes and supporting their postthaw development, which can assist with coral reef restoration.

珊瑚礁对全球海洋生态系统的健康与生物多样性至关重要，却因气候变化与人类活动出现了大幅衰退。超低温保存（Cryopreservation）指在超低温环境中长期保存生物材料的技术，是珊瑚保护领域的新兴策略。本研究探究了将特定脂质体掺入玻璃化溶液（vitrification solutions，简称VS）对两种珊瑚物种卵母细胞活力的影响：实验采用分别含有8种脂质之一的玻璃化溶液对卵母细胞进行玻璃化冷冻处理。研究结果显示，掺入磷脂酰乙醇胺（PE）与芥酸可显著提升J. juncea卵母细胞的活力，而掺入磷脂酰胆碱（PC）则会降低其活力；不过，掺入棕榈油酸、二十二碳六烯酸、亚油酸与二十碳三烯酸，并未对J. juncea卵母细胞的活力产生显著影响。对于J. fragilis而言，芥酸处理组的卵母细胞活力最高（54.12% ± 2.99%），油酸与二十二碳六烯酸处理组亦展现出积极效果。在所测试的脂质体中，芥酸在两种珊瑚物种中均实现了最高的综合活力水平。经脂质强化的玻璃化冷冻技术可通过助力珊瑚配子的超低温库构建，并支持其解冻后的发育过程，从而为珊瑚保护与珊瑚礁修复提供有力支撑。