The impact of different plant harvesting frequencies on microbial changes in Myriophyllum aquaticum wetlands

To investigate the impact of different harvesting frequencies on microbial changes in Myriophyllum aquaticum wetlands, three harvesting frequencies were set up: high harvesting frequency (45 day/ times), low harvesting frequency (LF: 90 day/ times) , and control (CK: no harvesting). Each wetland consisted of three consecutive units, referred to as CW1, CW2, and CW3, along the direction of water flow. The inlet water to the wetlands was low-concentration swine wastewater, and the hydraulic retention time for each wetland unit was 5 days. The surface sediment (0-5 cm) of the wetlands was used for microbial analysis. The meanings of the sample names used for microbial determination are as follows:(1) T1, T2, and T3 represent the CW1, CW2, and CW3 of M. aquaticum wetlands treated with high harvesting frequency in autumn, respectively.T4, T5, and T6 represent the CW1, CW2, and CW3 of M. aquaticum wetlands treated with low harvesting frequency in autumn, respectively.T7, T8, and T9 represent the CW1, CW2, and CW3 of M. aquaticum wetlands under control conditions in autumn, respectively.(2) T10, T11, and T12 represent the CW1, CW2, and CW3 of M. aquaticum wetlands treated with high harvesting frequency in winter, respectively.T13, T14, and T15 represent the CW1, CW2, and CW3 of M. aquaticum wetlands treated with low harvesting frequency in winter, respectively.T16, T17, and T18 represent the CW1, CW2, and CW3 of M. aquaticum wetlands under control conditions in winter, respectively.(3) T19, T20, and T21 represent the CW1, CW2, and CW3 of M. aquaticum wetlands treated with high harvesting frequency in spring, respectively.T22, T23, and T24 represent the CW1, CW2, and CW3 of M. aquaticum wetlands treated with low harvesting frequency in spring, respectively.T25, T26, and T27 represent the CW1, CW2, and CW3 of M. aquaticum wetlands under control conditions in spring, respectively.(4) T28, T29, and T30 represent the CW1, CW2, and CW3 of M. aquaticum wetlands treated with high harvesting frequency in summer, respectively.T31, T32, and T33 represent the CW1, CW2, and CW3 of M. aquaticum wetlands treated with low harvesting frequency in summer, respectively.T34, T35, and T36 represent the CW1, CW2, and CW3 of M. aquaticum wetlands under control conditions in summer, respectively.

为探究不同收割频率对狐尾藻（Myriophyllum aquaticum）湿地微生物群落变化的影响，本实验设置3种收割频率处理：高收割频率（每45天1次）、低收割频率（LF：每90天1次）以及对照组（CK：不收割）。每个湿地沿水流方向设有3个连续单元，分别记为CW1、CW2与CW3。湿地进水为低浓度生猪养殖废水，单个湿地单元的水力停留时间为5天。本实验选取湿地表层沉积物（0~5 cm）用于微生物群落分析。
用于微生物检测的样本名称含义如下：
(1) 秋季组：T1、T2、T3分别对应秋季高收割频率处理的狐尾藻湿地CW1、CW2与CW3单元；T4、T5、T6分别对应秋季低收割频率处理的狐尾藻湿地CW1、CW2与CW3单元；T7、T8、T9分别对应秋季对照组狐尾藻湿地CW1、CW2与CW3单元。
(2) 冬季组：T10、T11、T12分别对应冬季高收割频率处理的狐尾藻湿地CW1、CW2与CW3单元；T13、T14、T15分别对应冬季低收割频率处理的狐尾藻湿地CW1、CW2与CW3单元；T16、T17、T18分别对应冬季对照组狐尾藻湿地CW1、CW2与CW3单元。
(3) 春季组：T19、T20、T21分别对应春季高收割频率处理的狐尾藻湿地CW1、CW2与CW3单元；T22、T23、T24分别对应春季低收割频率处理的狐尾藻湿地CW1、CW2与CW3单元；T25、T26、T27分别对应春季对照组狐尾藻湿地CW1、CW2与CW3单元。
(4) 夏季组：T28、T29、T30分别对应夏季高收割频率处理的狐尾藻湿地CW1、CW2与CW3单元；T31、T32、T33分别对应夏季低收割频率处理的狐尾藻湿地CW1、CW2与CW3单元；T34、T35、T36分别对应夏季对照组狐尾藻湿地CW1、CW2与CW3单元。