Cellular Decoding of Simultaneous Cranial Mesenchyme and Osseous Architecture Reconstruction by Scaffolds

In this study, RNA sequencing was employed to investigate the differences in gene expression levels and patterns of cells recruited into suture-bony combined calvarial injury following the implatation of distinct types of commonly used calvarial scaffolds including gelatin methacryloyl hydrogel (GelMA), porous chitosan scaffold (CTS), and polylactic acid eletrospinning membrane (PLA). Nascent tissue from the suture-bony composite defects, where GelMA (3 samples), CTS (3 samples), PLA (2 samples), or no scaffold (NC, 3 samples) was implanted, were harvested 6 weeks after surgery. Flow cytometry was used to isolate Cd45- cells from digested tissue for RNA-seq.

本研究采用RNA测序技术，探究植入不同类型常用颅盖骨支架后，募集至颅缝-骨性复合颅盖损伤部位的细胞的基因表达水平与模式差异。所用支架包括甲基丙烯酸明胶水凝胶（GelMA）、多孔壳聚糖支架（CTS）以及聚乳酸静电纺丝膜（PLA）。术后6周，采集植入了GelMA（3份样本）、CTS（3份样本）、PLA（2份样本）或未植入支架（NC，3份样本）的颅缝-骨性复合缺损部位的新生组织。通过流式细胞术从消化后的组织中分离CD45阴性细胞，用于RNA测序（RNA-seq）。