Pseudomonadota Genome sequencing and assembly. Pseudomonadota

Bacterial genomes mutiplexed and sequenced using illumina Hiseq2000, we had more than 37 million pair of read, after QC, 9 million pair of read, all used for assembley ( clc_assembler 4.2.0). The result contigs/supercontigs have been filtered by removing contigs containing more than 15% run of Ns, and/or smaller than 1000b.

本研究的细菌基因组经多重建库测序后，采用Illumina HiSeq 2000（Illumina HiSeq 2000）测序平台完成测序，初始获得超过3700万条读对（read pair）。经质量控制（QC）过滤后，保留900万条读对，全部用于基因组组装，组装软件为CLC Assembler 4.2.0。最终得到的重叠群（contig）/超级重叠群（supercontig）需经过滤：移除包含超过15%连续N碱基，或长度小于1000 bp的序列。