Mus músculos domesticas Vkorc1 gene genotypes in the City of Barcelona

During the sampling period from 29/10/2018 to 31/01/2020, traps were installed inside the municipal facilities in the City of Barcelona. 111 Mus músculos domesticas simples were collected and a tail fragment was taken and stored at -20ºC for subsequent molecular analysis. We extracten genomic DNA and analyzed the mutations in the Vkorc1 gene in exon 1, exon 2 and exon 3 that are 174 bp, 110 bp and 202 bp long, respectively, and encode in total 161 amino acids.在2018年10月29日至2020年1月31日的采样周期内，研究人员于巴塞罗那市的市政设施内部布设了诱捕装置。共采集到111只小家鼠（Mus musculus domesticus），并截取其尾部组织片段保存于-20℃，用于后续分子分析。随后提取基因组DNA，对Vkorc1基因外显子1、外显子2与外显子3的突变情况进行分析；这三个外显子的长度分别为174 bp、110 bp及202 bp，总编码氨基酸数为161个。