MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network with PRC2 [RNA-seq]. MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network with PRC2 [RNA-seq]

We performed RNA-seq to identify differentially expressed genes in response to knockout of EZH2 and global miRNA knockdown (VP55 overexpressed) in T98G Glioblastoma multiforme cells. Overall design: RNA-seq in WT and EZH2-/- T98G cells and VP55 overexpressed WT and EZH2-/- T98G cells.

本研究通过RNA测序（RNA-seq），于T98G多形性胶质母细胞瘤（Glioblastoma multiforme）细胞中，鉴定响应EZH2基因敲除与全局microRNA（miRNA）敲低（通过过表达VP55实现）的差异表达基因。实验设计概述：对野生型（wild type, WT）、EZH2基因敲除型（EZH2-/-）T98G细胞，以及过表达VP55的野生型与EZH2基因敲除型T98G细胞进行RNA测序。