Gene expression patterns of a Rhodopseudomonas palustris ΔregSR strain under photoheterotrophic growth conditions. Gene expression patterns of a Rhodopseudomonas palustris ΔregSR strain under photoheterotrophic growth conditions

The redox-sensing two-component signal transduction system, RegSR, in Rhodopseudomonas palustris has been shown to regulate an uptake hydrogenase in response to varying cellular redox states; however, its role is still largely undefined. Here, we used RNA sequencing to compare gene expression patterns in wild type R. palustris strain CGA010 to a ΔregSR derivative, CGA2023, under varying metabolic conditions. Growth conditions were chosen to utilize the different metabolic capabilites of R. palustris and, thus, present a variety of different redox challenges to the cell. Overall design: Total RNA from two replicates of each strain was submitted for seven different growth conditions for a total of 28 samples. Cultures were provided with either high-light or low-light intensities in either nitrogen-fixing or non-nitrogen-fixing conditions. Carbon sources with varying redox states were provided including acetate, malate, and succinate as well as the aromatic compound benzoate.

沼泽红假单胞菌（Rhodopseudomonas palustris）中的氧化还原感应双组分信号转导系统RegSR已被证实可响应细胞内动态变化的氧化还原状态调控摄取氢酶的表达，但其具体生物学功能在很大程度上仍未明确。
本研究利用RNA测序（RNA sequencing）技术，在不同代谢条件下对比沼泽红假单胞菌野生型菌株CGA010与其ΔregSR缺失突变株CGA2023的基因表达谱。
本研究选取的培养条件旨在充分利用沼泽红假单胞菌的多样代谢能力，从而为细胞施加多种不同类型的氧化还原应激压力。
总体实验设计：每个菌株设置两个生物学重复，共覆盖7种不同培养条件，最终获得总计28份样本的总RNA用于测序。培养条件涵盖固氮与非固氮两种生长状态，同时设置高光与低光两种光照强度；碳源方面则选用具有不同氧化还原特性的乙酸盐、苹果酸盐、琥珀酸盐，以及芳香族化合物苯甲酸盐。