Defining the interplay of carbon dioxide and the carbonic anhydrase CanB in regulating Mycobacterium tuberculosis PhoPR signaling and virulence

Regulation of M. tuberculosis gene expression by carbon dioxide at acidic and neutral pH. Overall design: M. tuberculosis CDC1551 cultures were seeded at a starting OD600 of 0.2 in 8 mL of 7H9 buffered media and grown at 37°C in standing T-25 culture flasks. Two biological replicates of the following culture conditions were examined: i) 0.5% CO2 at pH 5.7, ii) 5% CO2 at pH 5.7, , iii) 0.5% CO2 at pH 7.0, iv) 5% CO2 at pH 7.0. Cultures were incubated for six days, after which total bacterial RNA was extracted.

酸性与中性pH条件下二氧化碳对结核分枝杆菌（M. tuberculosis）基因表达的调控。实验设计概述：将结核分枝杆菌CDC1551菌株的培养物以起始OD600为0.2的浓度接种至8mL经缓冲处理的7H9培养基中，于37℃静置培养于T-25培养瓶内。本实验针对以下四种培养条件各设置两个生物学重复：i）pH 5.7、0.5% CO₂环境；ii）pH 5.7、5% CO₂环境；iii）pH 7.0、0.5% CO₂环境；iv）pH 7.0、5% CO₂环境。所有培养物均孵育六天，随后提取细菌总RNA。