Comparative transcriptomics of Burkholderia pseudomallei from soil media and infected host

Burkholderia pseudomallei can adapt to and thrive in a variety of environments, including soil and water, and also can infect different hosts, including humans, leading to the tropical disease melioidosis. Modulation of gene and protein expression is one of this pathogen's adaptive survival mechanisms, which could lead to changes in the bacteria's cell membrane, metabolism, and virulence. To better understand bacterial adaptation and host-pathogen interactions, this study compared the expression profiles of B. pseudomallei from infected mice to B. pseudomallei cultivated in soil extract media. B. pseudomallei in vivo was created by infecting mice through the intraperitoneal route and harvesting the spleens on day 5 post infection. Total RNA was isolated and sequenced from the harvested spleen. Sequence reads were mapped to the B. pseudomallei UKMD286 strain genome sequence.

类鼻疽伯克霍尔德菌（Burkholderia pseudomallei）可适应并增殖于包括土壤、水体在内的多种环境中，同时能够感染包括人类在内的不同宿主，引发热带疾病类鼻疽（melioidosis）。基因与蛋白质表达的调控是该病原体适应性存活的机制之一，可引发细菌细胞膜、代谢过程及毒力发生改变。为更好地理解细菌适应性与宿主-病原体互作机制，本研究对比了感染小鼠体内的类鼻疽伯克霍尔德菌与土壤提取物培养基中培养的类鼻疽伯克霍尔德菌的表达谱。通过腹腔途径感染小鼠以获取体内的类鼻疽伯克霍尔德菌，并于感染后第5天采集脾脏。从采集的脾脏中分离总RNA并进行测序。将测序读数比对至类鼻疽伯克霍尔德菌UKMD286菌株的基因组序列。