Proteomic analysis of a clavata-like phenotype mutant in Brassica napus

Abstract Rapeseed is one of important oil crops in China. Better understanding of the regulation network of main agronomic traits of rapeseed could improve the yielding of rapeseed. In this study, we obtained an influrescence mutant that showed a fusion phenotype, similar with the Arabidopsis clavata-like phenotype, so we named the mutant as Bnclavata-like (Bnclv-like). Phenotype analysis illustrated that abnormal development of the inflorescence meristem (IM) led to the fused-inflorescence phenotype. At the stage of protein abundance, major regulators in metabolic processes, ROS metabolism, and cytoskeleton formation were seen to be altered in this mutant. These results not only revealed the relationship between biological processes and inflorescence meristem development, but also suggest bioengineering strategies for the improved breeding and production of Brassica napus.

摘要：油菜是我国重要的油料作物之一。深入解析油菜主要农艺性状的调控网络，可有效提升油菜产量。本研究中，我们获得了一株表现出融合表型的花序突变体，其表型与拟南芥类clavata表型相似，遂将该突变体命名为Bnclavata-like（Bnclv-like）。表型分析结果表明，花序分生组织（inflorescence meristem, IM）发育异常是引发该融合花序表型的根本原因。在蛋白质丰度层面，该突变体中参与代谢过程、活性氧（reactive oxygen species, ROS）代谢以及细胞骨架形成的核心调控因子均发生了显著改变。本研究结果不仅阐明了生物学过程与花序分生组织发育之间的内在联系，同时也为甘蓝型油菜（Brassica napus）的遗传改良育种与产业化生产提供了潜在的生物工程策略。