Analysis of the Microbial Community in Soil Fertilized with Palm Oil Mill Effluent

Palm oil derived from the fruits of Elaeis guineensis Jacq. has a global economic importance and is largely produced in tropical regions. Brazil is one of the main global producers. Palm oil production process lead to a highly polluting waste called Palm Oil Mill Effluent (POME). A strategy commonly used by producers to overcome environmental issues and to improve soil fertility is the reuse of POME as a fertilizer due to the chemical and biological characteristics of the effluent. In order to evaluate the impact of POME on soil bacterial diversity, three groups were analyzed: soil without POME application (control group - SP), and soil samples after 4 (P4) and 9 (P9) days of POME application. An environmental DNA metabarcoding approach was used. eDNA was extracted and the V4 region of the 16S rRNA gene was amplified and sequenced in the Illumina MiSeq platform.

由油棕（Elaeis guineensis Jacq.）果实制取的棕榈油具有重要的全球经济价值，其主产区为热带区域，巴西是全球主要生产国之一。棕榈油生产过程会产生一种污染极强的废弃物，称为棕榈油厂废水（Palm Oil Mill Effluent，POME）。鉴于该废水的化学与生物学特性，生产商常采用将POME作为肥料回用的策略，以解决环境问题并提升土壤肥力。为评估POME对土壤细菌多样性的影响，本研究设置三组样本进行分析：未施加POME的土壤（对照组SP）、施加POME后4天的土壤样本（P4组）以及施加POME后9天的土壤样本（P9组）。本研究采用环境DNA宏条形码技术，提取环境DNA（environmental DNA，eDNA），扩增16S核糖体RNA基因的V4区域，并通过Illumina MiSeq测序平台完成测序。