Direct regulation of shikimate, early phenylpropanoid and stilbenoid pathways by Subgroup 2 R2R3-MYBs in grapevine

V. vinifera cv. ‘Shiraz’ leaves from 10-week old plants were transformed via Agrobacterium-mediated infiltration with MYB15 (35S::VviMYB15) or an empty vector control. Samples were subsequently collected at five different time points (8h, 24h, 48h, 72h and 96h) in separate replicate pools (L1 and L2) consisting of three leaves from independent plants per time-point (n=2 biological replicates). Microarray analysis, using the Affymetrix V. vinifera Grape Array (A-AFFY-78), was carried out on extracted leaf RNA from three of the time points described for both control and MYB15 infiltrated samples. Across each time point, the two different pools of each condition (MYB15 vector and empty vector) were used as biological replicates.

以10周龄葡萄（Vitis vinifera）栽培品种‘西拉（Shiraz）’的叶片为实验材料，通过农杆菌介导的浸润法，分别转化携带35S启动子驱动的VviMYB15基因的载体（35S::VviMYB15）与空载体对照。随后在5个不同时间点（8h、24h、48h、72h、96h）采集样本，每个时间点设置独立的重复混合池（L1与L2），每个混合池包含3株独立植株的叶片，共设置2次生物学重复（n=2）。针对上述对照组与MYB15浸润组的样本，选取其中3个时间点的叶片提取总RNA，采用Affymetrix葡萄基因组芯片（Affymetrix V. vinifera Grape Array，编号A-AFFY-78）开展微阵列分析。每个时间点下，两种处理组（MYB15载体组与空载体组）的两个独立混合池均作为生物学重复样本。