RNAseq of pancreata from KRasG12D/RosaMycER treated with Tamoxifen for different times

Experiment designed to study the effect of deregulated myc on pancreatic cancer progression and regression. In the KRasG12D/RosaMycER mouse model KRas and MycER are expressed exclusively in the pancreas, but MycER activity depends on Tamoxifen presence. RNAseq was preformed on pancreata from mice treated with Tamoxifen as follows: 1- Normal food. 2- Tamoxifen food 2 weeks. 3- Tamoxifen food 2 weeks, followed by normal food 1 day. 4- Tamoxifen food 2 weeks, followed by normal food 3 days.

本实验旨在探究失调的Myc基因（myc）对胰腺癌进展与消退的影响。在KRasG12D/RosaMycER小鼠模型中，KRas与MycER仅在胰腺组织中表达，而MycER的活性依赖于他莫昔芬（Tamoxifen）的存在。本研究对接受以下他莫昔芬处理方案的小鼠胰腺组织进行了RNA测序（RNA-seq）：
1. 正常饮食组；
2. 他莫昔芬饮食喂养2周组；
3. 他莫昔芬饮食喂养2周后，更换为正常饮食1天组；
4. 他莫昔芬饮食喂养2周后，更换为正常饮食3天组。