Detection of cellulose production capacity of recombinant Escherichia coli strains BL21(DE3) and AAEC191A

Bacteria that form biofilms generate an extracellular matrix (ECM), where cellulose stands out as a key constituent. An approach for assessing microorganisms' cellulose production involves using calcofluor white staining on colonies. In this method, a fluorescent dye (calcofluor-white) is introduced to a stable YESCA substrate composed of casamino acids, agar, and yeast extract. The fluorescent dye specifically attaches to cellulose, enabling bacterial colonies to be observed under ultraviolet light. The study employed YESCA medium supplemented with calcofluor-white, as well as YESCA + 0.2% glucose medium supplemented with calcofluor-white.
The dataset contains original photos of bacterial colonies of the following E. coli strains: BL21(DE3)/pCC90, BL21(DE3)/pACYCpBAD, BL21(DE3)/pCC90 Dra D-mut, BL21(DE3)/pCC90 D54-STOP AAEC191A/pCC90, AAEC191A/pACYCpBAD, AAEC191A/pCC90 Dra D-mut, AAEC191A/pCC90 D54-STOP, BL21(DE3), AAEC191A and CFT073. Bacteria were cultured in YESCA medium with the addition of calcofluor-white at a temperature of 25OC and 37OC  for 5 days. Photographs of the colony were taken at one-day intervals.

形成生物膜的细菌会产生细胞外基质（extracellular matrix，ECM），其中纤维素是关键组分。评估微生物纤维素产生能力的一种方法是使用钙荧光白（calcofluor white）对菌落进行染色。在此方法中，将荧光染料钙荧光白（calcofluor-white）加入由酪蛋白氨基酸、琼脂和酵母提取物组成的稳定YESCA底物中。该荧光染料特异性结合纤维素，使细菌菌落可在紫外光下观察。本研究采用添加钙荧光白的YESCA培养基，以及添加钙荧光白的YESCA+0.2%葡萄糖培养基。
该数据集包含以下大肠杆菌（E. coli）菌株的原始菌落照片：BL21(DE3)/pCC90、BL21(DE3)/pACYCpBAD、BL21(DE3)/pCC90 Dra D-mut、BL21(DE3)/pCC90 D54-STOP、AAEC191A/pCC90、AAEC191A/pACYCpBAD、AAEC191A/pCC90 Dra D-mut、AAEC191A/pCC90 D54-STOP、BL21(DE3)、AAEC191A和CFT073。细菌在添加钙荧光白的YESCA培养基中，于25℃和37℃条件下培养5天，每隔一天拍摄菌落照片。