Identification of the role of tumor intrinsic Malat1 in regulation of immune microenvironment [scRNA-seq]

We have injected 4T1-TGL breast cancer cells into Balbc mice through tail vein. In order to see immune cells infiltration, we have isolated CD45+ cells from these mice lungs at different time points by FACS. These isolated cells were further subjected for scRNA-seq. For base line we have isolated CD45+ cells from Naive balbc mice. Lung CD45+ cells were isolated for scRNA-seq and sent for sequencing at MDACC Sequencing Core Facilities. The sample name was annotated below. SC78_1: Naïve SC78_2: WT-D10 SC78_3: KO-D10 SC78_6:WT-D20 SC78_7: KO-D20 SC78_9: WT-D30 SC78_10: KO-D30

本研究通过尾静脉向BALB/c小鼠注射4T1-TGL乳腺癌细胞。为探究免疫细胞浸润情况，本研究通过流式细胞术（FACS）于不同时间点从造模小鼠肺部分离CD45阳性（CD45+）细胞，并将分离得到的细胞进一步开展单细胞RNA测序（scRNA-seq）。本研究同时从未造模BALB/c小鼠体内分离CD45+细胞作为基线对照。所有用于单细胞RNA测序的肺部CD45+细胞均送至MDACC测序核心设施完成测序。样本命名及对应信息如下：SC78_1：未造模基线组；SC78_2：野生型造模后第10天组（WT-D10）；SC78_3：基因敲除型造模后第10天组（KO-D10）；SC78_6：野生型造模后第20天组（WT-D20）；SC78_7：基因敲除型造模后第20天组（KO-D20）；SC78_9：野生型造模后第30天组（WT-D30）；SC78_10：基因敲除型造模后第30天组（KO-D30）