Additional file 1 of Super enhancer related gene ANP32B promotes the proliferation of acute myeloid leukemia by enhancing MYC through histone acetylation

Additional file 1. Figure S1. Western blotting analysis showed that ANP32B protein levels were downregulated in MV4-11 and Kasumi-1 cells after BRD4 knockdown. Figure S2. The knockdown level of ANP32B in MV4-11 and Kasumi-1 cells was verified by qPCR. Figure S3. The knockdown of ANP32B inhibited the growth of MV4-11 cells. A. Monitoring of body weight of the two groups of mice. B. Representative images of H&E staining analysis of liver in two groups of mice. C. Representative images of IHC staining of mice liver. Figure S4. The knockdown of ANP32B inhibited the growth of Kasumi-1 cells. A. Different size and weight of spleen, from sh-NC or sh-ANP32B mices. B. Representative images of IHC staining of mice spleen. Figure S5. IGV visual analysis showed a reduction in H3K27ac signaling in genes involved in the MYC signaling pathway. Figure S6. ANP32B is positively correlated with C-MYC expression. A. Western blotting analysis showed that ANP32B overexpression was established successfully. B. Western blotting analysis showed that ANP32B was positively correlated with C-MYC expression. Table S1. The primer sequences used in this study. Table S2. Super-enhancers identified in each of the 11 AML samples. Table S3. Deferentially expressed genes identified by RNA-Seq of MV4-11 cell after ANP32B knockdown. Table S4. Deferentially expressed genes identified by RNA-Seq of Kasumi-1 cell after ANP32B knockdown. Table S5. In the control group, peaks called by ChIP-Seq of H3K27ac in MV4-11 cell. Table S6. In the ANP32B knockdown group, peaks called by ChIP-Seq of H3K27ac in MV4-11 cell.

补充文件1。
补充图S1：蛋白质免疫印迹（Western blotting）分析显示，BRD4敲低后的MV4-11与Kasumi-1细胞中，ANP32B蛋白表达水平显著下调。
补充图S2：通过实时定量聚合酶链反应（qPCR）验证了MV4-11与Kasumi-1细胞内ANP32B的敲低效率。
补充图S3：敲低ANP32B可抑制MV4-11细胞的增殖。A. 两组小鼠的体重监测结果；B. 两组小鼠肝脏苏木精-伊红（H&E）染色的代表性图像；C. 两组小鼠肝脏免疫组化（IHC）染色的代表性图像。
补充图S4：敲低ANP32B可抑制Kasumi-1细胞的增殖。A. sh-NC组与sh-ANP32B组小鼠的脾脏大小及重量差异；B. 两组小鼠脾脏免疫组化（IHC）染色的代表性图像。
补充图S5：整合基因组浏览器（IGV）可视化分析显示，MYC信号通路相关基因的H3K27ac信号强度显著降低。
补充图S6：ANP32B与C-MYC的表达呈正相关。A. 蛋白质免疫印迹（Western blotting）分析证实ANP32B过表达细胞模型构建成功；B. 蛋白质免疫印迹（Western blotting）分析显示ANP32B的表达与C-MYC的表达呈显著正相关。
补充表S1：本研究使用的引物序列。
补充表S2：11例急性髓系白血病（AML）样本中分别鉴定得到的超级增强子（super-enhancers）。
补充表S3：对ANP32B敲低后的MV4-11细胞进行RNA测序（RNA-Seq）后鉴定得到的差异表达基因。
补充表S4：对ANP32B敲低后的Kasumi-1细胞进行RNA测序（RNA-Seq）后鉴定得到的差异表达基因。
补充表S5：对照组中，对MV4-11细胞进行H3K27ac染色质免疫共沉淀测序（ChIP-Seq）所得到的测序峰。
补充表S6：ANP32B敲低组中，对MV4-11细胞进行H3K27ac染色质免疫共沉淀测序（ChIP-Seq）所得到的测序峰。