RNA-seq of in vitro differentiated naïve and Th9 cells

To investigate changes in gene expression over time, we differentiated Th9 cells in vitro from naïve T cells from human and murine sources, and followed them over an extended time course, during which they gained and then lost capacity for IL-9 production We then performed gene expression profiling analysis using data obtained from RNA-seq in cells from 2 different human donors and from 2 mice at 4 time points. Overall design: Comparative gene expression profiling analysis of RNA-seq data for d0 (naïve), d3, d4. and d8 Th9 cells (mouse) or d0 (naïve), d5, d8, and d15 Th9 cells (human)

为探究基因表达随时间的动态变化规律，我们分别从人类和小鼠来源的初始T细胞（naïve T cells）中体外诱导分化为Th9细胞（Th9 cells），并对其开展长期时序追踪：在此过程中，这些细胞产生白细胞介素9（IL-9）的能力先升后降。随后，我们针对来自2名人类供体与2只小鼠的细胞，以RNA测序（RNA-seq）获取的表达数据为基础，开展基因表达谱分析，共设置4个时间采样点。本研究的整体实验设计为：对小鼠Th9细胞的d0（初始态）、d3、d4、d8，以及人类Th9细胞的d0（初始态）、d5、d8、d15的RNA测序数据进行对比基因表达谱分析。