Psychotria homalosperma genome sequencing. Psychotria homalosperma

Genome sequencing of Psychotria homalosperma was performed by using Roche 454 reads. Specifically, total genomic DNA of Psychotria homalosperma was extracted from fresh leaf collected from Sekimon (26?40'11.3"N 142?09'16.4"E) on Hahajima Island of the Bonin Islands, using the DNasy Plant Mini Kit. The extracted DNA of P. homalosperma was pyrosequenced using a 454 GS Junior System. Multiplex Identifier (MID) tags were used for multiplexing of this sample and another species in the Bonin Islands, then both samples were mixed. Row data were demultiplexed and MID tags removed from the reads using Newbler.

本研究针对密花九节（Psychotria homalosperma）开展基因组测序，测序基于罗氏454（Roche 454）读长序列完成。具体而言，研究人员从位于小笠原群岛（Bonin Islands）母岛（Hahajima Island）关门（Sekimon）地区（坐标：26°40'11.3"N 142°09'16.4"E）采集的新鲜叶片中提取该物种的总基因组DNA，提取过程使用DNasy植物迷你试剂盒（DNasy Plant Mini Kit）。所提取的密花九节DNA采用454 GS Junior测序系统进行焦磷酸测序。研究使用多重标识符（Multiplex Identifier，MID）标签对该样本与小笠原群岛的另一物种样本进行多重测序标记，随后将两份样本混合。使用Newbler软件对原始测序数据进行解多重标记处理，并从读长序列中移除MID标签。