A staged approach using ants as test organism in ecotoxicology: Data repository

Ants (Hymenoptera: Formicidae) are eusocial insects and essential for maintaining biodiversity and ecosystem functioning. Despite their ecological relevance, ants are often neglected in ecotoxicity testing. We developed a staged testing scheme using ants as non-target organisms by exposing workers, larvae, and founding queens of three ant species (<i>Camponotus maculatus</i>, <i>Crematogaster</i> sp., and <i>Lasius niger</i>) to various concentrations of the neonicotinoid imidacloprid. Sub-colonies were orally exposed to honey water - imidacloprid solutions and lethal and sublethal effects were monitored and are presented in detail.This Data Repository provides the raw data used for the anaylses in Pohl et al. 2025 (A staged approach using ants as test organism in ecotoxicology)

蚂蚁（膜翅目Hymenoptera：蚁科Formicidae）是真社会性昆虫（eusocial insects），对维持生物多样性与生态系统功能至关重要。尽管其生态意义重大，但在生态毒理学（ecotoxicology）测试中常被忽视。我们开发了一套以蚂蚁作为非靶标生物（non-target organisms）的阶段性测试方案，将3种蚂蚁（Camponotus maculatus、Crematogaster sp.以及Lasius niger）的工蚁、幼虫以及创始蚁后（founding queens）暴露于不同浓度的新烟碱类（neonicotinoid）杀虫剂吡虫啉（imidacloprid）中。实验中亚群经口暴露于蜂蜜水-吡虫啉混合溶液中，研究人员对其致死效应与亚致死效应（sublethal effects）进行了监测，并将结果详细呈现。本数据集仓库提供了Pohl等人2025年发表的论文《生态毒理学中以蚂蚁作为测试生物的阶段性方法》中分析所用的原始数据。