Transcriptomic study of the impact of iron toxicity on rice plant. Oryza sativa

transcriptomic study of the impact of iron toxicity on rice plant (Oryza sativa L.; cv ‘I Kong Pao’ ) after short term (3 days) or long term (3 weeks) exposure to ferrous iron (125 ppm). Twenty five days old rice seedlings were exposed to 0 or 125 mg/L ferrous iron for 3 days and 3 weeks in hydroponic culture. Comparison between control and iron stressed plants were done at the shoot and the root levels. The assays were replicated twice on two independent plant cultures. Overall design: 8 samples, Two-condition experiment, control (0 ppm ferrous iron) vs. iron treated (125 ppm ferrous iron). Biological replicates: 2 replicates for comparison shoot 3 days of stress, root 3 days of stress, shoot 3 weeks of stress and root 3 weeks of stress.

本研究为针对二价铁（125 ppm）短期（3天）或长期（3周）暴露对水稻（Oryza sativa L.；品种‘I Kong Pao’）铁毒性影响的转录组学研究。实验采用水培培养体系，将25日龄水稻幼苗分别暴露于0 mg/L与125 mg/L的二价铁环境中，处理时长分别为3天与3周。本研究对对照组与铁胁迫组水稻的地上部及根系样本开展比较分析，实验在两批独立的水稻培养体系中完成两次重复。
实验总体设计：共包含8个样本，为双条件对照实验，即对照组（0 ppm二价铁）与铁处理组（125 ppm二价铁）。生物学重复设置：针对3天胁迫地上部、3天胁迫根系、3周胁迫地上部以及3周胁迫根系这四组比较样本，每组均设置2次生物学重复。