Epicardial Hdac3 promotes myocardial growth through a novel microRNA pathway [mRNA-seq]. Epicardial Hdac3 promotes myocardial growth through a novel microRNA pathway [mRNA-seq]

The purpose of the study was to compare epicardial transcript abundance between control and Hdac3 knockout (KO) epicardial cells (MEC). Overall design: Total RNA extracted from CTL (EV) and Hdac3 CRISPR/CAS9 knockout (KO) in mouse epicardial cells. RNA libraries were prepared and Illumina HiSeq NGS preformed (Novogene Corporation Inc, Sacramento, CA) per standard protocols. For experiments with a biological replicate, as the DESeq2 has already eliminated the biological variation, our threshold is normally set to the following, padj < 0.05.

本研究旨在对比对照组与组蛋白去乙酰化酶3（HDAC3）敲除（KO）小鼠心外膜细胞（MEC）的转录本丰度。
实验设计：从对照组（CTL，EV组）及经CRISPR/CAS9介导的组蛋白去乙酰化酶3敲除的小鼠心外膜细胞中提取总RNA，按照标准实验流程构建RNA文库，并采用Illumina HiSeq平台开展下一代测序（NGS），测序服务由美国加利福尼亚州萨克拉门托的诺禾致源公司（Novogene Corporation Inc）提供。
对于包含生物学重复的实验，鉴于DESeq2已完成生物学变异的校正，本研究的显著性筛选阈值通常设定为校正后P值（padj）<0.05。