Quantitative proteomic dataset of the moss Physcomitrium patens SMG1 KO mutant line

Here, we provide an isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomic dataset of the moss Physcomitrium patens smg1 knockout line. The kinase SMG1 is one of the key components of the NMD system in many organisms, including plants. Several lines with a deleted SMG1 in a land Physcomitrium patens subsp. patens (“Gransden 2004”, Frieburg) were produced by James P. B. Lloyd [doi.org/10.1111/tpj.12329]. One of these lines, SMG1 KO mutant line 2, was used for this study. Protonemata of WT and mutant lines were grown in200 ml liquid BCD medium supplemented with 5 mM ammonium tartrate (BCDAT) during a 16-h photoperiod at 250C for 8 days [https://doi.org/10.1101/pdb.prot5136]. All experiments were performed in three biological replicates.

本数据集为基于同量异位素相对与绝对定量（isobaric tag for relative and absolute quantitation，iTRAQ）标记的定量蛋白质组学数据集，对应材料为苔藓小立碗藓（Physcomitrium patens）SMG1基因敲除株系。激酶SMG1是包括植物在内的诸多生物中无义介导mRNA降解（nonsense-mediated mRNA decay，NMD）系统的核心组分之一。James P. B. Lloyd创制得到了陆生小立碗藓原亚种（Physcomitrium patens subsp. patens，材料编号"Gransden 2004"、"Frieburg"）的多个SMG1基因敲除株系[DOI:10.1111/tpj.12329]。本研究选用其中的SMG1敲除突变株系2号开展实验。将野生型（wild type，WT）及突变株系的原丝体接种于200 mL添加有5 mM酒石酸铵的液体BCD培养基（BCDAT）中，于16小时光照周期、25℃条件下培养8天[DOI:10.1101/pdb.prot5136]。所有实验均设置三次生物学重复。