Transcriptome of Tomato (Solanum lycopersicum) Reveals Impacts of Root Microbiomes on Host Interactions (Tomato plant RNA sequences)

In this project, we sought to assess the effects of microbiomes derived from vermicompost on tomato productivity and physiology. We cultivated tomatoes in a greenhouse with three distinct microbial inoculants: one created from vermicompost (V), one created from heat treated vermicompost (HT), and one created from sterilized luria broth (LB). We then sequenced the rhizosphere microbiome to compare community composition between inoculant treatments, performed plant phenotyping to compare productivity between treatments, and performed transcriptomics on foliar and fruit tissue to assess the effects of microbial treatments on tomato hosts at the molecular level.

本研究旨在探究源自蚯蚓粪（vermicompost）的微生物组对番茄生产力与生理特性的影响。我们在温室中栽培番茄，并设置三种不同的微生物菌剂（microbial inoculants）处理组：分别为蚯蚓粪来源菌剂（V组）、热处理蚯蚓粪来源菌剂（HT组），以及灭菌LB培养基（sterilized luria broth, LB）来源菌剂（LB组）。随后，我们对根际微生物组（rhizosphere microbiome）进行测序，以比较不同菌剂处理组间的群落组成；开展植物表型分析，以对比各组处理的番茄生产力；并对叶片与果实组织进行转录组学（transcriptomics）分析，以从分子水平评估微生物菌剂处理对番茄宿主的影响。