Expression data from autophagy-deficient keratinocytes and normal keratinocytes. Expression data from autophagy-deficient keratinocytes and normal keratinocytes

There is increasing evidence that autophagy contributes to the epidermal differentiation; however, the role of autophagy in epidermal tight junction (TJ) barrier remains unclear. To evaluate the role of autophagy in the maintenance of skin TJ barrier, we knocked out autophagy in human primary keratinocytes by infecting cells with autophagy-related gene 3 (Atg3) C264S mutant adenovirus. Overall design: A total of 4 samples were analyzed and data from autophagy-deficient keratinocytes and normal keratinocytes were compared.

越来越多的证据表明，细胞自噬（autophagy）参与表皮分化过程，但细胞自噬在表皮紧密连接（TJ）屏障中的作用仍不明确。为探究细胞自噬在皮肤TJ屏障维持中的作用，本研究通过用自噬相关基因3（Atg3）C264S突变腺病毒感染人原代角质形成细胞，敲除了细胞的自噬功能。总体实验设计：本研究共分析4组样本，对自噬缺陷型角质形成细胞与正常角质形成细胞的相关数据进行了对比分析。