Paramecium tetraurelia PacBio RSII sequencing T50 stage

Aims: Characterizing the distribution of 6mA (6-methyladenine) in Paramecium tetraurelia. Materials & methods: Total genomic DNA was extracted from wild type Paramecium tetraurelia cells (strain 51 mating type 7) at a late developmental stage (T50) of the self-fertilization process of autogamy. 2 SMRTbell libraries were prepared (size selection step using a 15kb (Library 1) or 13kb (Library 2) cutoff) then were sequenced on 16 SMRTcells on the RSII instrument. 6mA were detected and mapped using the ipdSummary.py script from SMRT-v2.3.0. Results: Our results revealed enrichment of 6mA into clusters separated by a periodic distance downstream Transcription Start Sites in the somatic genome.

研究目的：表征四膜虫（Paramecium tetraurelia）中6-甲基腺嘌呤（6-methyladenine，简称6mA）的分布特征。
材料与方法：从处于自体受精（autogamy）过程晚期发育阶段（T50时期）的野生型四膜虫（品系51，交配型7）细胞中提取全基因组DNA。构建2个SMRTbell文库，其中文库1以15kb为长度筛选阈值，文库2以13kb为长度筛选阈值；随后在RSII测序仪的16个SMRT细胞上完成测序。使用SMRT-v2.3.0版本配套的ipdSummary.py脚本完成6mA的检测与基因组定位。
研究结果：本研究结果显示，在体细胞基因组中，6mA富集于转录起始位点（Transcription Start Sites）下游以周期性间隔分隔的簇状区域内。