Frequency of NK progenitors in EB and ES-HP subpopulations

†Progenitor frequency was determined by sorting limiting numbers of cells into 96 well plates containing OP9 stroma and media for NK differentiation cultures as described in materials and methods. NK cells were detected by granzyme B RT-PCR.*Derived from CD34+ EB cells.**Derived from CD34+CD45− EB cells.#Average of two independent experiments. All other values are average of three independent experiments.

† 祖细胞频率的测定方式如下：参照材料与方法中的描述，将有限稀释数目的细胞分选至含有OP9基质细胞与NK细胞分化培养基的96孔板中进行培养；通过颗粒酶B RT-PCR检测自然杀伤（NK）细胞。
* 源自CD34阳性（CD34+）胚状体（embryoid body, EB）细胞。
** 源自CD34阳性、CD45阴性（CD34+CD45−）胚状体（EB）细胞。
# 该组数据为两次独立实验的平均值；其余所有数值均为三次独立实验的平均值。