Dynamics of m6A RNA methylome during leukemogenesis reveals a role of PRMT6-MFSD2A signaling axis in maintaining AML stem cells.[Chip-seq]

We delineate a comprehensive m6A landscape during acute myeloid leukemia (AML) development, and identify PRMT6, regulated by IGF2BP2, acting as a key for leukemia stem cell maintenance by restraining MFSD2A expression and docosahexaenoic acid level. Overall design: H3R2me2a ChIP-seq of Prmt6-/- and Igf2bp2-/- leukemia cells

我们描绘了急性髓系白血病（acute myeloid leukemia, AML）发生进程中的完整m6A修饰图谱，并鉴定出受IGF2BP2调控的PRMT6，其通过抑制MFSD2A的表达并降低二十二碳六烯酸（docosahexaenoic acid）水平，成为维持白血病干细胞（leukemia stem cell）的关键调控因子。实验设计概述：针对Prmt6基因敲除（Prmt6⁻/⁻）与Igf2bp2基因敲除（Igf2bp2⁻/⁻）的白血病细胞，开展H3R2me2a染色质免疫共沉淀测序（ChIP-seq）实验。