The Role of Histone H3 Lysine 36 Methylation in Reprogramming of fibroblasts and on Induced Pluripotent stem Cell Generation

SETD2 downregulation via two independent shRNA infection significantly modulated expression profile of dH1f cells in preOSKM and postOSKM samples such that 186/121 genes were down and 135/66 were up-regulated in preOSKM, and 467/544 were down and 313/586 were up-regulated in postOSKM cells significantly (padj<0.001 and log2 fold change>0.5). Overall design: Examination of SETD2 downregulation effect on gene expression profile of dH1f cells in pre- and 6 days postOSKM samples. 2 sets of samples were prepared for shSETD2-1/shSETD2-3 preOSKM and 3 sets of samples were prepared for shCntrl preOSKM, shSETD2-1/ shSETD2-3 and shCntrl postOSKM conditions.

通过两种独立的短发卡RNA（short hairpin RNA，shRNA）感染介导SETD2基因下调，可显著调控dH1f细胞在OSKM诱导前（preOSKM）与OSKM诱导后6天的样本（postOSKM）中的基因表达谱：在preOSKM样本中，分别有186/121个基因显著下调、135/66个基因显著上调；在postOSKM细胞中，分别有467/544个基因显著下调、313/586个基因显著上调，上述差异均满足校正后P值（padj）<0.001且log2倍变化（log2 fold change）>0.5的筛选标准。整体实验设计：本研究旨在探究SETD2下调对dH1f细胞在preOSKM及postOSKM样本中的基因表达谱的影响。样本制备情况如下：针对shSETD2-1/shSETD2-3感染的preOSKM样本，制备了2组样品；针对短发卡RNA对照（shCntrl）preOSKM、shSETD2-1/shSETD2-3及shCntrl感染的postOSKM样本，制备了3组样品。