miRNA profiling in primary human peritoneal mesothelial cells (HPMCs) and TGF-β1-stimulated

TORAY microarray assays were performed on HPMCs derived from four independent donors. miRNA expression was measured in control samples and after 1 ng/ml of TGF-β1 treatment for 48 h. TGF-β1-driven mesothelial-to-mesenchymal transition (MMT) was confirmed in all samples by analysis of the eight molecular markers previously described (data not shown). A total of 1 μg in a volume of ≤2 μl of total RNA from each sample was subsequently used for Toray microarray analysis, performed by Central Biotechnology Services at Cardiff University. miRNA expression was measured in control samples and after 1 ng/ml of TGF-β1 treatment for 48 h. TGF-β1-driven mesothelial-to-mesenchymal transition (MMT) was confirmed in all samples by analysis of the eight molecular markers previously described (data not shown).

本研究针对源自4名独立供体的人腹膜间皮细胞（Human Peritoneal Mesothelial Cells, HPMCs）开展东丽（TORAY）微阵列实验。我们对对照组样本以及经1 ng/ml转化生长因子β1（Transforming Growth Factor-β1, TGF-β1）处理48小时后的样本中的微小RNA（microRNA, miRNA）表达水平进行了检测。通过对既往已报道的8种分子标志物进行分析（数据未展示），证实所有样本均发生了转化生长因子β1诱导的间皮-间质转化（mesothelial-to-mesenchymal transition, MMT）。随后，我们取每份样本中总量为1 μg、体积≤2 μl的总RNA，用于东丽微阵列分析，该实验由卡迪夫大学中央生物技术服务中心完成。我们再次对对照组样本以及经1 ng/ml转化生长因子β1处理48小时后的样本中的微小RNA表达水平进行了检测。通过对既往已报道的8种分子标志物进行分析（数据未展示），证实所有样本均发生了转化生长因子β1诱导的间皮-间质转化。