Raw Data of Activity of Lactobacillus gasseri strain LLG1 and its exopolysaccharides in inflammatory cell model and on Candida albicans proliferation

These data correspond to the raw data from the article "Activity of Lactobacillus gasseri strain LLG1 and its exopolysaccharides in inflammatory cell model and on Candida albicans proliferation" (final version). They correspond to the original files for Figures 1, 2, 3, 4, 5, Figures 1, 2 and 3 Supplementary data from the article, which aimed to observe by confocal microscopy the co-incubation of Lactobacillus gasseri and Candida albicans, each labelled with DAPI and Concanavalin A-rhodamine, respectively; and to verify the purity of the exopolysaccharides obtained, in particular the absence of protein in the exopolysaccharide extractions by SDS-PAGE and Bradford assay, or the absence of LTA by Western blot, and a negligible amount of nucleic acids by spectrophotometer (DeNovix DS-11). The data include images obtained from the filamentation of Candida albicans in the presence of Lg-EPS using a Leica DMI8, as well as confocal microscopy images of Candida albicans, Lactobacillus gasseri, or both microorganisms co-incubated for several hours, and the original images obtained by confocal microscopy or those derived from SDS-PAGE and Western blot analyses. The data include raw data used for the relative expression of pro-inflammatory cytokines (from Caco-2 cells and macrophages) and inflammatory markers by qPCR, as well as the protein levels secreted by macrophages in the presence of L. gasseri or their EPS (Lg-EPS) by ELISA. The data also includes mRNA purity measured by spectrophotometer (DeNovix DS-11) and to assess the quality of our RNA, the RNA 6000 Pico Kit for the 2100 Bioanalyzer system was used (Agilent). The data also include analysis of AhR activity induced by Lg-EPS. In parallel, MTT analyses highlight the effects of Lg-EPS on the proliferation of Caco-2 cells. Some of the results obtained on the effects of Lg-EPS on C. albicans are also presented via biofilm by Crystal Violet, fungal adhesion on Caco-2 cells (via Calcein AM) and by absorbance measurements of Candida growth in the presence of Lg-EPS.

本数据集对应发表于论文"《一株格氏乳杆菌（Lactobacillus gasseri）及其胞外多糖在炎症细胞模型中的活性及对白色念珠菌（Candida albicans）增殖的影响》"的原始数据，包含该论文中图1、2、3、4、5的源文件，以及图1、2、3的补充数据。本研究旨在通过共聚焦显微镜（confocal microscopy）观察分别经4',6-二脒基-2-苯基吲哚（DAPI）和刀豆球蛋白A-罗丹明（Concanavalin A-rhodamine）标记的格氏乳杆菌与白色念珠菌的共孵育情况；同时验证所提取胞外多糖的纯度，具体包括通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和布拉德福德法（Bradford assay）检测胞外多糖提取物中无蛋白质残留、通过蛋白质免疫印迹（Western blot）检测无脂磷壁酸（LTA）残留、通过超微量紫外分光光度计（Nanodrop）检测无核酸残留。本数据集包含在Lg-EPS（格氏乳杆菌胞外多糖）作用下白色念珠菌菌丝形成的相关图像，以及白色念珠菌、格氏乳杆菌单独或二者共孵育数小时后的共聚焦显微镜图像，同时包含共聚焦显微镜拍摄的原始图像，以及源自SDS-PAGE和蛋白质免疫印迹分析的衍生图像。本数据集包含用于分析Caco-2细胞促炎细胞因子相对表达量及炎症标志物的原始数据、计算过程与统计结果（采用实时定量聚合酶链反应（qPCR）检测），还包含通过酶联免疫吸附测定（ELISA）检测的巨噬细胞在格氏乳杆菌或其胞外多糖（Lg-EPS）作用下分泌的蛋白水平数据。本数据集还包含通过超微量紫外分光光度计检测的mRNA纯度数据；为评估RNA质量，本研究使用了适配2100生物分析仪系统的安捷伦（Agilent）RNA 6000 Pico试剂盒（货号：5067-1513）。本数据集同时包含Lg-EPS诱导的芳香烃受体（AhR）活性分析数据。此外，噻唑蓝（MTT）分析法验证了Lg-EPS对Caco-2细胞增殖的影响。本数据集还包含通过结晶紫染色法检测Lg-EPS对白色念珠菌生物膜影响的相关结果，以及在Lg-EPS存在下检测白色念珠菌生长情况的吸光度测量数据。