Establish the male meiocyte-specific epigenomic landscape. Establish the male meiocyte-specific epigenomic landscape

Histone modification maps of H3K4me3 and H3K27me3 of meiocytes were obtained by microlibrary and high-throughput sequencing by extracting meiocytes from rice varieties Azucena and IR64. And the DNA methylation results of meiocytes and flag-leaf of Azucena and IR64 were obtained. By collating this data, the male meiocyte-specific epigenomic landscape is constructed Overall design: By micromanipulation technology, the meiocytes of Azucena and IR64 were obtained, respectively. Libraries were constructed by CUT&Tag technology to obtain histone modification data. DNA methylation libraries were constructed by scBS-seq technology. In addition, flag-leaf's DNA methylated library is constructed by directly extracting whole genome DNA

本研究从水稻品种Azucena和IR64中分离减数分裂细胞，通过微文库构建与高通量测序，获取了这些减数分裂细胞的H3K4me3与H3K27me3组蛋白修饰图谱；同时还获取了Azucena与IR64的减数分裂细胞及剑叶的DNA甲基化数据。通过整合上述数据，构建了雄性减数分裂细胞特异性表观基因组图谱。
总体实验设计：采用显微操作技术分别分离得到Azucena与IR64的减数分裂细胞；利用CUT&Tag技术构建文库以获取组蛋白修饰数据；采用单细胞亚硫酸氢盐测序（single-cell bisulfite sequencing, scBS-seq）技术构建DNA甲基化文库；此外，通过直接提取全基因组DNA的方式，构建了剑叶的DNA甲基化文库。