Characteristic dissection of Xanthomonas oryzae pv. oryzae responsive microRNAs in rice [datatset 1]

we emphatically monitored the responsive changes of genes targeted by rice miRNAs miRNAs at 0, 8, 24 hours across Xoo strain PXO86 infection in its compatible rice variety IR24 and incompatible variety IRBB5 by degradome technology.These findings provide new insights into the complex roles of characteristic miRNAs in rice-Xoo interactions. Overall design: To recognize the post-transcriptional regulatory units caused by miRNAs during rice-Xoo interaction, two mixed degradome libraries, IR24-D(including IR24-0 h, IR24-8 h and IR24-24 h) and IRBB5-D(including IRBB5-0 h, IRBB5-8 h and IRBB5-24 h), were generated, which could provide the valuable experimental target genes validation through detecting the cleavage sites detection of miRNAs.

本研究采用降解组测序技术（degradome technology），重点监测了亲和性水稻品种IR24与非亲和性水稻品种IRBB5在受到Xoo菌株PXO86侵染后的0、8、24小时时，其体内水稻microRNA（miRNA）靶向基因的响应变化。本研究结果为解析水稻与Xoo互作过程中特征miRNA的复杂调控作用提供了全新视角。整体实验设计：为阐明水稻与Xoo互作过程中miRNA介导的转录后调控单元，我们构建了两组混合降解组文库：IR24-D（包含IR24-0 h、IR24-8 h与IR24-24 h三个样本）与IRBB5-D（包含IRBB5-0 h、IRBB5-8 h与IRBB5-24 h三个样本）。通过检测miRNA的靶基因剪切位点，可为相关靶基因的实验验证提供极具价值的依据。