RNA-seq analysis of HeLa cells in nutrient-rich and nutrient-deprived media

RNA-seq analysis was applied in two independent batches, each one in triplicate samples of HeLa cells in nutrient-rich and nutrient-deprived media Overall design: Transcriptome analysis of HeLa cells in nutrient-rich and nutrient-deprived (starved) media in two independent batches/sets, each one in three biological repeats

本研究采用RNA测序（RNA-seq）技术开展分析，共设置两个独立实验批次，每个批次均包含三份HeLa细胞重复样本，分别培养于营养丰富培养基与营养匮乏培养基中。实验设计：针对营养丰富及营养匮乏（饥饿处理）条件下培养的HeLa细胞进行转录组分析，实验分为两个独立批次/组别，每个批次设置三次生物学重复。