Long non-coding RNA maternally expressed 3 (MEG3) regulates isoflurane-induced cognitive dysfunction by targeting miR-7-5p

This study aimed to investigate the role and mechanism of long non-coding RNA maternally expressed gene 3 (MEG3) in cognitive dysfunction induced by isoflurane (ISO). Morrier water maze analysis was performed to evaluate the cognitive function of rats. Modified modified neurological severity score (mNSS) scores were assessed for neurological damage. The levels of MEG3 in hippocampal tissues of rats and hippocampal neuron cell lines HT22 were examined by reverse transcription-quantitative polymerase chain reaction (qRT-PCR). Moreover, the cell viability and apoptosis were assessed by the Cell Counting Kit-8 (CCK-8) and flow cytometry assay. Indicators of inflammation and oxidative stress were determined using enzyme-linked immunosorbent assay (ELISA) and commercial assay kits. Relationship between MEG3 and microRNA (miR)-7-5p was verified by the dual-luciferase reporter gene assay. MEG3 was increased in hippocampal tissues and HT22 after ISO treatment (p p p

本研究旨在探讨长链非编码RNA母系表达基因3（MEG3）在异氟烷（ISO）诱导的认知功能障碍中的作用及机制。采用莫里斯水迷宫评估大鼠的认知功能，采用改良神经功能缺损评分（mNSS）评估神经损伤程度。通过逆转录定量聚合酶链反应（qRT-PCR）检测大鼠海马组织及海马神经元细胞系HT22中MEG3的表达水平。此外，采用细胞计数试剂盒-8（CCK-8）检测细胞活力，通过流式细胞术检测细胞凋亡水平；采用酶联免疫吸附试验（ELISA）及商用检测试剂盒测定炎症与氧化应激相关指标。通过双荧光素酶报告基因试验验证MEG3与微小RNA（miR）-7-5p之间的靶向调控关系。异氟烷处理后，大鼠海马组织及HT22细胞中MEG3的表达水平升高（p p p）