Additional file 8 of TGF-β1 dominates stromal fibroblast-mediated EMT via the FAP/VCAN axis in bladder cancer cells

Additional file 8: (A) Immunofluorescence shows that the fluorescence intensity of VCAN in HFFs is markedly enhanced under the induction of TGF-β1; TGF-β1 neutralizing antibody significantly reduces this enhanced fluorescence intensity. (B) qRT-PCR verifies that the expression of VCAN in OEVCAN-HFFs is significantly higher than in NCVCAN-HFFs and T24 cells. (C) Immunofluorescence reveals that the VCAN fluorescence intensity in OEVCAN-HFFs is notably brighter than in NCVCAN-HFFs and T24 cells. (D) ELISA shows that the concentration of VCAN in the OEVCAN-HFF supernatant is significantly higher than in NCVCAN-HFF and T24 cell supernatant. (E) WB show that OEVCAN-HFF-CM can downregulate E-cadherin as well as upregulate N-cadherin and Vimentin.

附加文件8：(A) 免疫荧光（Immunofluorescence）结果显示，在转化生长因子-β1（TGF-β1）诱导下，人包皮成纤维细胞（HFFs）中VCAN的荧光强度显著增强；TGF-β1中和抗体可显著降低该增强的荧光信号。(B) 实时定量反转录聚合酶链反应（qRT-PCR）验证结果表明，OEVCAN-HFFs中VCAN的表达水平显著高于NCVCAN-HFFs与T24细胞。(C) 免疫荧光（Immunofluorescence）结果显示，OEVCAN-HFFs中的VCAN荧光强度显著高于NCVCAN-HFFs与T24细胞。(D) 酶联免疫吸附实验（ELISA）结果表明，OEVCAN-HFF细胞上清液中的VCAN浓度显著高于NCVCAN-HFF与T24细胞上清液。(E) 蛋白质免疫印迹（Western Blot，WB）结果显示，OEVCAN-HFF条件培养基可下调E-钙粘蛋白（E-cadherin）的表达，同时上调N-钙粘蛋白（N-cadherin）与波形蛋白（Vimentin）的表达。