Hepatic gene expression data from A/J mouse and A/J-12SM consomic mouse. Mus musculus

SMXA-5 mouse shows a high-fat induced fatty liver. The QTL for fatty liver was mapped on mouse chromosome 12, designated as Fl1sa. The liver triglycerides content of A/J-12SM consomic mouse, a chromosome substitution strain, was suppressed compared to that of A/J mouse. We compared the hepatic gene expressions between A/J mouse and A/J-12SM consomic mouse. Overall design: Total RNA of male A/J mice and A/J-12SM consomic mice fed a high-fat diet for 7weeks were extracted individually from liver of nine mice. The RNAs from three mice were pooled and hybridized on one Affimetrix microarray. Sample were analyzed in triplicate set of array (three biological replicates).

SMXA-5小鼠呈现高脂诱导的脂肪肝表型。脂肪肝相关的数量性状基因座（quantitative trait locus, QTL）被定位至小鼠12号染色体，命名为Fl1sa。A/J-12SM染色体替换系小鼠的肝脏甘油三酯含量相较于A/J小鼠受到显著抑制。本研究对A/J小鼠与A/J-12SM染色体替换系小鼠的肝脏基因表达谱进行了比较分析。

总体实验设计：对饲喂高脂饲料7周的雄性A/J小鼠及A/J-12SM染色体替换系小鼠，从9只个体的肝脏中分别提取总RNA。将每3只小鼠的RNA混合为一个样本，随后在一张Affymetrix微阵列上完成杂交。本实验设置三次生物学重复，即共完成三组独立的芯片杂交分析。