Transcriptome analysis of the D3 mouse embryonic stem cell line in comparison to the MIN6 pancreatic beta-cell insulinoma cell line

Analysis of the transcriptional differences between mouse ES cells from the D3 cell line and transformed beta-cells (MIN6 cell line) as determined by a direct comparative analysis of their transcriptome. The results show that 40% of transcripts were differentially expressed between D3 and MIN6 cells. There is thus a marked difference in the pattern of transcription between the ES cell and beta-cell genomes. Total RNA was extracted from three independent replicates of passage 21 D3 cells and passage 23 MIN6 cells. A whole mouse genome comparison between D3 and MIN6 cells was performed using the Mouse WG-6 v2.0 Expression Beadchip from Illumina.

本研究通过转录组直接比较分析，对D3细胞系来源的小鼠胚胎干细胞（Embryonic Stem Cell, ES）与转化型β细胞（MIN6细胞系）之间的转录差异展开探究。分析结果显示，D3细胞与MIN6细胞间有40%的转录本存在差异表达，由此可见胚胎干细胞与β细胞的基因组转录模式存在显著差异。研究人员从第21代D3细胞与第23代MIN6细胞的3组独立生物学重复样本中提取总RNA，并采用伊卢米纳（Illumina）公司的Mouse WG-6 v2.0表达微珠芯片，完成了两类细胞的全小鼠基因组表达谱比较分析。