Proteomic characterization of Rathayibacter toxicus FH 79 using LC MS/MS

Rathayibacter toxicus is a gram-positive bacterium that is the causative agent of annual ryegrass toxicity, a disease that causes devastating losses in the Australian livestock industry. This bacterium is poorly characterized, making it difficult to accurately detect in feed samples. Using 1-D gels and mass spectrometry, we analyzed the protein expression of R. toxicus under stationary growth phase conditions to obtain a more complete understanding of the mechanisms of this organism. A total of 333 unique proteins were identified. The data obtained in this analysis is an essential first step toward developing an antibody-based diagnostic assay.

有毒拉土杆菌（Rathayibacter toxicus）是一种革兰氏阳性（Gram-positive）细菌，为一年生黑麦草中毒症的病原菌，该病害曾给澳大利亚畜牧产业造成毁灭性损失。当前对该细菌的研究尚不充分，导致难以在饲料样本中实现精准检测。本研究采用一维凝胶电泳（1-D gels）与质谱（mass spectrometry）技术，分析了R. toxicus在稳定生长期的蛋白质表达情况，以全面解析该病原菌的致病机制。本次分析共鉴定得到333种独特蛋白质。本分析所获数据是开发基于抗体的诊断检测方法的关键第一步。