QPCTL deletion results in a rewiring of the tumor microenvironment [scRNA-seq]. QPCTL deletion results in a rewiring of the tumor microenvironment [scRNA-seq]

single cell RNA sequencing performed on the FACS sorted tumor microenvironment of (QPCTL-proficient and -deficient) B16F10 tumors Overall design: C57/Bl6JR mice that were QPCTL-WT or QPCTL-KO were innoculated with either QPCTL-proficient or deficient B16F10 tumor cells, respectively (n=3 each). After 14 days tumors were harvested, single cell suspensions were made and live cells were isolated by FACS, after which cells were processed for scRNA-seq (10X Chromium 3' kit), and sequenced on Illumina NextSeq

本研究针对经荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）分离的QPCTL功能正常型与功能缺陷型（QPCTL-proficient and -deficient）B16F10肿瘤的肿瘤微环境，开展单细胞RNA测序（single cell RNA sequencing, scRNA-seq）。实验设计：将QPCTL野生型（QPCTL-WT）与QPCTL基因敲除型（QPCTL-KO）的C57/Bl6JR小鼠分别接种对应基因型的QPCTL功能正常型及功能缺陷型B16F10肿瘤细胞，每组各3个生物学重复。接种14天后获取肿瘤组织，制备单细胞悬液并通过FACS分离活细胞，随后采用10X Chromium 3'试剂盒处理细胞以进行scRNA-seq，并在Illumina NextSeq测序平台完成测序。