Terminal uridyltransferase 7 regulates TLR4-triggered inflammation by controlling Regnase-1 mRNA uridylation and degradation. Terminal uridyltransferase 7 regulates TLR4-triggered inflammation by controlling Regnase-1 mRNA uridylation and degradation

We revealed that the transcriptome of the mammalian cells under inflammatory status using RNA Sequencing (RNA-Seq). The transcriptome of BMDMs from wild-type and Tut7-/- was compared to baseline. The fold-change was presented by the logarithm of p-values to the base 2 and the significance was presented by the negative logarithm of that to the base 10. log2FC > +0.2 and log2FC 20% and decreased by >20%, respectively. Among the 352 genes, 111 of them involving in innate immune response were downregulated in Tut7-/- cells. Overall design: LPS-induced BMDMs mRNA profiles of 7-week-old wild type and Tut7-/- mice were generated by deep sequencing, in duplicate, using Illumina HiSeq PE150

本研究通过RNA测序（RNA Sequencing, RNA-Seq）解析了炎症状态下哺乳动物细胞的转录组。对野生型与Tut7-/-小鼠的骨髓源性巨噬细胞（Bone Marrow-Derived Macrophages, BMDMs）的转录组与基线状态进行了比对分析。本研究以2为底的倍数变化对数（log₂FC）表示差异倍数，以该值的10为底负对数表示统计学显著性；其中log₂FC > +0.2的基因上调幅度超过20%，log₂FC < -0.2的基因下调幅度超过20%。在筛选得到的352个基因中，有111个参与先天免疫应答（innate immune response）的基因在Tut7-/-细胞中呈下调表达。整体实验设计：采用Illumina HiSeq PE150测序平台，对7周龄野生型及Tut7-/-小鼠的脂多糖（Lipopolysaccharide, LPS）诱导的骨髓源性巨噬细胞进行双重复深度测序，以获取其mRNA表达谱。