Single cell RNA sequencing reveals a cardioprotective role for resident macrophages following myocardial infarction (microarray). Single cell RNA sequencing reveals a cardioprotective role for resident macrophages following myocardial infarction (microarray)

Human left ventricular cardiac tissue was isolated from patients at the time of surgery. Tissue was digested into single cell suspension and labeled with CD45, CD14, HLA-DR and CCR2 antibodies. Macrophages were sorted into three groups (CCR2+ Monocytes: CD14+ CCR2+ HLA-DRNeg; CCR2+ Macrophages: CD14+CCR2+HLA-DR+; MHC-II hi Macrophages: CD14+CCR2-HLA-DR+) Overall design: RNA was extracted from each subset for Gene array analysis

本研究的人类左心室心肌组织均取自手术术中的患者离体标本。将组织消化为单细胞悬液，并用CD45、CD14、HLA-DR及CCR2抗体进行标记。巨噬细胞经分选分为三个亚群：CCR2阳性单核细胞（CCR2+ Monocytes）：CD14+ CCR2+ HLA-DRNeg；CCR2阳性巨噬细胞（CCR2+ Macrophages）：CD14+ CCR2+ HLA-DR+；MHC-II高表达巨噬细胞（MHC-II hi Macrophages）：CD14+ CCR2- HLA-DR+。实验整体设计：从每个细胞亚群中提取RNA以进行基因芯片分析。