MSC stimulate ovarian tumor growth during intercellular communication but reduce tumorigenicity after fusion with ovarian cancer cells. MSC stimulate ovarian tumor growth during intercellular communication but reduce tumorigenicity after fusion with ovarian cancer cells

Functional changes were investigated in vitro and in vivo following spontaneous fusion and hybrid cell formation in co-cultures of primary human mesenchymal stroma/stem-like cells (MSC) with human SK-OV-3 ovarian cancer cells. Lentiviral fluorescence-labeled MSC with eGFP and ovarian cancer cells labeled with mcherry resulted in dual-fluorescing hybrid cells. Double FACS sorting and single cell cloning revealed two different aneuploid hybrid populations (SK-hyb1 and SK-hyb2) Overall design: A total of 4 different total RNA samples was subjected to microarray analysis according to a single-color design.

本研究针对原代人类间充质基质/干细胞样细胞（primary human mesenchymal stroma/stem-like cells, MSC）与人类SK-OV-3卵巢癌细胞的共培养体系中，自发融合及杂交细胞形成后的功能变化，开展了体外与体内实验探究。经慢病毒荧光标记并携带增强绿色荧光蛋白（enhanced green fluorescent protein, eGFP）的间充质干细胞，以及经mCherry标记的卵巢癌细胞，可形成双荧光阳性杂交细胞。通过双重荧光激活细胞分选（fluorescence-activated cell sorting, FACS）与单细胞克隆，成功获得两种不同的非整倍体杂交细胞群（SK-hyb1与SK-hyb2）。实验整体设计：共选取4份不同的总RNA样本，采用单通道微阵列设计开展基因芯片分析。