A natural plant derived compound, Withaferin A exploits the stress pathway to induce antitumor immunity

Analysis of the global effect of plant derived compound Withaferin A on gene expression in rat her-2/neu expressing murine cancer cells at an early time point of 6hrs post treatment. Pathway analyses were performed to determine which metabolic pathways were significantly affected by the Withaferin A. Total RNA was isolated from rat her-2/neu cells treated with Withaferin A for 6hrs or untreated cells.

本研究分析了植物来源化合物睡茄内酯A（Withaferin A）在处理后6小时这一早期时间点下，对表达大鼠her-2/neu基因的小鼠癌细胞基因表达的全局影响。通过通路分析，明确睡茄内酯A显著影响的代谢通路。分别从经睡茄内酯A处理6小时的大鼠her-2/neu细胞与未处理对照细胞中提取总RNA。