Transcriptome analysis of alternative splicing under ABA exposure in osfkbp20-1b mutant compared to wild-type (ZH11) of rice

To investigate whether observed changes in pre-mRNA splicing led to OsFKBP20-1b depletion, we analyzed RNA-seq to identify the quantitative profiling and alternative splicing Overall design: RNA was extracted from osfkbp20-1b and WT of 1 week rices and treated with mock or 100 µM ABA for 6 hr

为探究观测到的前体mRNA（pre-mRNA）剪接变化是否引发OsFKBP20-1b表达耗竭，我们通过RNA测序（RNA-seq）分析进行定量表达谱与可变剪接的鉴定。
实验总体设计：从1周龄水稻的osfkbp20-1b突变体与野生型（WT）材料中提取RNA，分别以Mock对照或100 μM ABA（脱落酸）处理6小时。