Gene expression profiling from cohesin knockdown cells obtained grown in methylcellulose

We performed RNA-sequencing in c-Kit+ cells that were infected with retroviruses expressing shRNAs for Renilla, Rad21, Smc1a, Smc3 or Stag2. These cells were grown in methylcellulose (M3434) for either one passage (P1) or replated for five passages (P5). RNA-sequencing control (Ren) and cohesin (Rad21, Smc1a, Smc3 and Stag2) knockdown cells.

我们对经携带靶向Renilla、Rad21、Smc1a、Smc3或Stag2的短发夹RNA（shRNA）的逆转录病毒感染的c-Kit阳性细胞开展了RNA测序（RNA-sequencing）。将上述细胞置于甲基纤维素培养基（M3434）中，分别培养至1次传代（P1）或复传5次（P5）。本研究的RNA测序样本涵盖两类：RNA测序对照细胞（Ren，即Renilla组），以及黏连蛋白（cohesin）组分Rad21、Smc1a、Smc3与Stag2基因敲低的细胞。